Human Vitamin B12 CLIA Kit


Human Vitamin B12 CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Vitamin B12 in serum.

​​​​​​​For research use only. Not for use in diagnostics procedures.


The Human Vitamin B12 CLIA Kit is designed, developed, and produced for the quantitative measurement of human VB 12 level in serum samples. The assay utilizes competition law for testing. The calibrators, controls, or sample, monoclonal antibody to B12 labeled with microparticles are mixed and incubated, forming an antigen-antibody immuno-complex. Acridinium ester labeled B12 are added and incubated.Acridinium ester labeled B12 unbound to the sample binds to the Magnetically microgranular B12 antibody. After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of B12 present in the sample. The test result is automatically calculated by the system according to the working curve.​​​​​


Catalog no. SKT-070R
Target Human Vitamin B12
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 30.00 pg/mL
Dynamic Range 30.00 pg/mL~2000 pg/mL
Total Incubation Time 15 minutes
Sample Type Serum
Sample Volume 34 µL
Storage Temperature 2-8 °C

Selected Literature

1.Miller, Ariel; Korem, Maya; Almog, Ronit; Galboiz, Yanina (June 15, 2005). "Vitamin B12, demyelination, remyelination and repair in multiple sclerosis". Journal of the Neurological Sciences. 233 (1-2): 93–97.
2.Yamada, Kazuhiro (2013). "Chapter 9. Cobalt: Its Role in Health and Disease". In Astrid Sigel, Helmut Sigel and Roland K. O. Sigel.Interrelations between Essential Metal Ions and Human Diseases.Metal Ions in Life Sciences. 13. Springer. pp. 295–320.
​​​​​​​3. Martens, J. H., Barg, H., Warren, M., &Jahn, D. (2002). Microbial production of vitamin B12.Applied microbiology and biotechnology, 58(3), 275-285.
For research use only. Not for use in diagnostics procedures.