The Human Folate CLIA Kit is designed, developed, and produced for the quantitative measurement of human Folate level in serum samples. The assay utilizes competitive binding mechanisms for testing. The calibrators, controls, or sample, Anti-biotin coupled to paramagnetic particles and Anti-folate labeled with biotin are mixed and incubated, forming an antigen-antibody immunocomplex. Folate labeled with acridinium ester were added and incubated. Anti-folate labeled with biotin unbound to the sample binds to the Folate labeled with acridinium ester, and then binds to the microparticles through reactions with biotin. After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of folate present in the sample. The test result is automatically calculated by the system according to the working curve.