SKT-060R

Human Thyroglobulin CLIA Kit

Description


Human Thyroglobulin CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human thyroglobulin concentration in serum.
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​​​​​​​For research use only. Not for use in diagnostics procedures.

Background


The Human Thyroglobulin CLIA Kit is designed, developed, and produced for the quantitative measurement of human TG level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of TG.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti-TG polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin TG antibody–TG–acridinium ester TG antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a TG in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum TG concentration.

Specifications

Catalog no. SKT-060R
Target Human thyroglobulin
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.100 ng/mL
Dynamic Range 0.100 ng/mL~500.0 ng/mL
Total Incubation Time
Sample Type Serum
Sample Volume 20 µL
Storage Temperature 2-8 °C

Selected Literature

  1. Boron WF (2003). Medical Physiology: A Cellular And Molecular Approaoch. Elsevier/Saunders. p. 1044. ISBN 1-4160-2328-3.
  2. Wild D. The Immunoassay Handbook-the theory and applications of ligand binding, ELISA and related techniques, Fourth Edition.Elsevier Ltd. 2013.
  3. Pacini F, Schlumberger M, Dralle H, et al. European consensus for the management of patients with differentiated thyroid carcinoma of the follicular epithelium. Eur J Endocrinol 2006; 154:787–803.
For research use only. Not for use in diagnostics procedures.