Human Insulin CLIA Kit

Description

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Background

The Human Insulin CLIA Kit is designed, developed, and produced for the quantitative measurement of human Insulin level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Insulin.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti-Insulin polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin Insulin antibody–Insulin–acridinium ester Insulin antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a Insulin in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum Insulin concentration.

Selected Literature

​​​​​​​1. Dimitriadis G, Mitrou P, Lambadiari V, et al. Insulin effects in muscle and adipose tissue. Diabetes Research and Clinical Practice, 2011, 93 (1): S52-S59.
2. Steiner DF, Oyer PE. The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma. Proc Natl Acad Sci USA, 1967, 57 (2): 473–480.

For research use only. Not for use in diagnostics procedures.