SKT-044

Human Anti-Thyroglobulin Antibody CLIA Kit

Description

Human Anti-Thyroglobulin Antibody CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Anti-Thyroglobulin Antibody concentration in serum.
​​​​​​​
For in-vitro diagnostics purposes only

Background


The Human Anti-Thyroglobulin Antibody(Anti-Tg) CLIA Kit is designed, developed, and produced for the quantitative measurement of human Anti-Tg level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Anti-Tg.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti-Tg polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin Tg antibody–Tg–acridinium ester Tg antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a Tg in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum Tg concentration.

Specifications

Catalog no. SKT-044
Target Human Anti-Thyroglobulin Antibody
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 10.00 IU/mL
Dynamic Range 10.00 IU/mL~4000 IU/mL
Total Incubation Time
Sample Type Serum
Sample Volume 20 µL
Storage Temperature 2-8 °C

Selected Literature


1.Mansourian AR. Metabolic pathways of tetraidothyronine and triidothyronine production by thyroid gland: a review of articles. Pak J Biol Sci, 2011, 14(1): 1-12.
2. Ruf J, Ferrand M, Durand-Gorde JM, et al. Significance of thyroglobulin antibodies cross-reactive with thyroperoxidase (TGPO antibodies) in individual patients and immunized mice. Clin Exp Immunol, 1993, 92(1): 65-72.

For in-vitro diagnostic use.