Human Growth Hormone CLIA Kit

Description

​​​​​​​Human Growth Hormone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human growth hormone concentration in serum.

Background

The Human growth hormone CLIA Kit is designed, developed, and produced for the quantitative measurement of human hGH level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of hGH.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti- hGH polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin hGH antibody–hGH–acridinium ester hGH antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a hGH in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum hGH concentration.

Selected Literature

​​​​​​​1. Devesa J, Devesa P, Reimunde P. Growth hormone revisited. Med Clin (Barc), 2010, 135(14): 665-670.
2. Doga M, Bonadonna S, Gola M, et al. Growth hormone deficiency in the adult. Pituitary, 2006, 9:305-311.
3. Iranmanesh A, Grisso B, Veldhuis JD. Low basal and persistent pulsatile growth hormone secretion are revealed in normal and hyposomatotropic men studied with a new ultrasensitive chemiluminescence assay. J Clin Endocrinol Metab, 1994, 78: 526-535.

For in-vitro diagnostic use.