Total Triiodothyronine CLIA Kit


​​​​​​​Total Triiodothyronine CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human total Triiodothyronine concentration in serum.
For in-vitro diagnostics purposes only


The total Triiodothyronine CLIA Kit is designed, developed, and produced for the quantitative measurement of human T3 level in serum samples. The assay utilizes competition law for testing.
The calibrators, controls, or sample, acridinium ester labeled T3 monoclonal antibody are mixed and incubated, forming an antigen-antibody immunocomplex. Monoclonal T3 antigen coupled to paramagnetic particles T3 are added and incubated. Acridinium ester labeled T3 monoclonal antibody unbound to the sample binds to the Magnetically microgranular T3, After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of T3 present in the sample. The test result is automatically calculated by the system according to the working curve.


Catalog no. SKT-040
Target Human total Triiodothyronine
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.250 ng/mL
Dynamic Range 0.2 ng/mL~8.0 ng/mL
Total Incubation Time 10 Minutes
Sample Type Serum
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature

​​​​​​​1.Larsen PR. Triiodothyronine: Review of Recent Studies of Its Physiology and Pathophysiology in Man. Metabolism 1972; 21: 1073-1092.
2.Klee GG. Clinical usage recommendations and analytic performance goals for total and free triiodothyronine
​​​​​​​3. measurements. Clinical Chemistry 1996;42:155–159.
For in-vitro diagnostic use.