Total Thyroxine CLIA Kit


Total Thyroxine CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human total Thyroxine in serum.
​​​​​​​For research use only. Not for use in diagnostics procedures.


The T4 CLIA Kit is designed, developed, and produced for the quantitative measurement of human T4 level in serum samples. The assay utilizes competition law for testing. The assay utilizes competitive binding mechanisms for testing.The calibrators, controls, or sample, acridinium ester labeled T4 monoclonal antibody are mixed and incubated, forming an antigen-antibody immunocomplex. Monoclonal T4 antigen coupled to paramagnetic particles T4 are added and incubated. Acridinium ester labeled T4 monoclonal antibody unbound to the sample binds to the Magnetically microgranular T4, After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of T4 present in the sample. The test result is automatically calculated by the system according to the working curve.​​​​​​


Catalog no. SKT-039R
Target Human total Thyroxine
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 2.50 ng/mL
Dynamic Range 2.5 ng/mL to 300.0 ng/mL
Total Incubation Time 10 Minutes
Sample Type Serum
Sample Volume 15 µL
Storage Temperature 2-8 °C

Selected Literature

​​​​​​​1.Wenzel KW. Pharmacological interference with in vitro tests of thyroid function. Metabolism 1981;30(7):717–732
2. Burrow GN. Thyroid status in normal pregnancy. J ClinEndocrinolMetab 1990;71:274–275
3. Lazarus JH, Othman S. Thyroid disease in relation to pregnancy. ClinEndocrinol 1991; 34:91–98.
For research use only. Not for use in diagnostics procedures.