Free Triiodothyronine CLIA Kit


This Free Triiodothyronine CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Free Triiodothyronine concentration in serum.
​​​​​​​For research use only. Not for use in diagnostics procedures.


The Human fT3 CLIA Kit is designed, developed, and produced for the quantitative measurement of human fT3 level in serum samples. The assay utilizes competition law for testing.
The calibrators, controls, or sample, monoclonal antibody to fT3 labeled with microparticles are mixed and incubated, forming an antigen-antibody immuno-complex. Acridinium ester labeled fT3 are added and incubated.Acridinium ester labeled fT3 unbound to the sample binds to the Magnetically microgranular fT3 antibody. After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of fT3 present in the sample. The test result is automatically calculated by the system according to the working curve.​​​​​​


Catalog no. SKT-038R
Target Human Free Triiodothyronine
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 2.0 pmol/L
Dynamic Range 2.0 pmol/L to 50.0 pmol/L
Total Incubation Time 10 Minutes
Sample Type Serum
Sample Volume 25 µL
Storage Temperature 2-8 °C

Selected Literature

1.KaplanMM.Assessment of Thyroid Function During Pregnancy.Thyroid 1992;2(2):57-61
​​​​​​​2.Keffer JH. Preanalytical considerations in testing thyroid function.ClinChem 1996;42(1):125-134 3.Klee GG.Clinical usage recommendations and analytic performance goals for total and free triiodothyroninemeasurements.ClinChem 1996;42(1):155-159
For research use only. Not for use in diagnostics procedures.