Pro-Gastrin-Releasing Peptide CLIA Kit

Description

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Background

The Pro-Gastrin-Releasing Peptide CLIA Kit is designed, developed, and produced for the quantitative measurement of Pro-gastrin-releasing peptide (ProGRP) level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of ProGRP. Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the ProGRP in the form of “magnetic particles–ProGRP antibody–ProGRP– acridinium ester ProGRP antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a ProGRP in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum ProGRP concentration.

Selected Literature

​​​​​​​​​​​​​​1. Ischia J, Patel O, Shulkes A, et al. Gastrin-releasing peptide: different forms, different functions[J]. Biofactors, 2009, 35(1):69–75 .
2. Schneider J, Philipp M, Salewski L, et al. Pro-gastrinreleasing peptide (ProGRP) and neuron specific enolase (NSE) in therapy control of patients with small-cell lung cancer.[J]. Clinical Laboratory, 2003, 49(2):35-42.
​​​​​​​3.Miyake Y,Kodama T,Yamaguchi K.Pro—gastrin releasing peptide(31-98)is a specific tumor marker in patients with small cell lung carcinoma.[J] Cancer Research,1994:54:2136-2140.

For research use only. Not for use in diagnostics procedures.