Human Squamous Cell Carcinoma antigen CLIA Kit


Human Squamous Cell Carcinoma Antigen CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Squamous Cell Carcinoma concentration in serum.
For in-vitro diagnostics purposes only


​​​​​​​The Human Squamous Cell Carcinoma antigen CLIA Kit is designed, developed, and produced for the quantitative measurement of human SCC levels in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of SCC. Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the SCC in the form of “magnetic particles–SCC antibody–SCC–acridinium ester SCC antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a SCC in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum SCC concentration.


Catalog no. SKT-030
Target Human Squamous Cell Carcinoma
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD ≤0.200 ng/mL
Dynamic Range 0.200 ng/mL to 70.0 ng/mL
Total Incubation Time 15 minutes
Sample Type Serum
Sample Volume 20 µL
Storage Temperature 2-8 °C

Selected Literature

​​​​​​​1.Chen Y M, Wang L, Zhong L H, et al. Application of tumor markers in the early diagnosis of ovarian cancer (J). Current Medicine,2012; 18 (20) : 12-3.
​​​​​​​2.Chen Jing-jing, Chen Jian-min, Sun Quan. Clinical significance of CEA, CK19 and SCC in diagnosis of lung cancer [J]. Modern immunology, 2017,37 (3) : 22.
3.Zhu Lin,GUOGuang-hong,Evaluation of ChemiluminescentMicroparticle Immunoassay for Detection of Squamous Cell Carcinoma Antigen,Labeled Immunoassays and Clinical Medicine,2016, 23(10).
For in-vitro diagnostic use.