SKT-029R

Human Carcinoembryonic Antigen CLIA Kit

Description

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Human Carcinoembryonic Antigen CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Carcinoembryonic Antigen concentration in serum.

For research use only. Not for use in diagnostics procedures.

Background


​​​​​​​The Human CEA CLIA Kit is designed, developed, and produced for the quantitative measurement of human CEA level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of CEA
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti- CEA polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin CEA antibody–CEA–acridinium ester CEA antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a CEA in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum CEA concentration.

Specifications

Catalog no. SKT-029R
Target Carcinoembryonic Antigen
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD ≤0.2ng/mL.
Dynamic Range 0.2-1000 ng/mL
Total Incubation Time
Sample Type Serum
Sample Volume 20 µL
Storage Temperature 2-8 °C

Selected Literature


​​​​​​​1.Chen Y M, Wang L, Zhong L H, et al. Application of tumor markers in the early diagnosis of ovarian cancer (J). Current Medicine,2012; 18 (20) : 12-3.
2.Chen Jing-jing, Chen Jian-min, Sun Quan. Clinical significance of CEA, CK19 and SCC in diagnosis of lung cancer [J]. Modern immunology, 2017,37 (3) : 22.​​​​​​
For research use only. Not for use in diagnostics procedures.