Anti-thyroid peroxidase antibody CLIA Kit


​​​​​​​Anti-thyroid peroxidase antibody CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of Anti-thyroid peroxidase antibody concentration in serum.
For in-vitro diagnostics purposes only


The Anti-thyroid peroxidase antibody CLIA Kit is designed, developed, and produced for the quantitative measurement of Anti-TPO level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Anti-TPO.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti-Anti-TPO polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin Anti-TPO antibody–Anti-TPO–acridinium ester Anti-TPO antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a Anti-TPO in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum Anti-TPO concentration.



Catalog no. SKT-027
Target Anti-thyroid peroxidase antibody
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.50 IU/L
Dynamic Range 0.50 IU/mL to 1000.0 IU/mL
Total Incubation Time
Sample Type Serum
Sample Volume 20 µL
Storage Temperature 2-8 °C

Selected Literature

1.Boscato Lm, Stuart MC. Heterophilic antibodies: a problem for all immunoassays. Clin Chem 1988; 34:27-33.
2.Kricka L. Interferences in immunoassays - still a threat. Clin Chem 2000; 46: 1037–1038.
3.Bjerner J, et al. Immunometric assay interference: incidence and prevention. Clin Chem 2002; 48: 613–621.

For in-vitro diagnostic use.