SKT-025

Human Procalcitonin CLIA Kit

Description

​​​​​​​Human Procalcitonin CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Procalcitonin concentration in serum.

For in-vitro diagnostics purposes only

Background


The Human Procalcitonin (PCT) CLIA Kit is designed, developed, and produced for the quantitative measurement of human PCT level in serum samples. The assay utilizes a twosite “sandwich” technique with two antibodies that bind to different epitopes of PCT. Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the PCT in the form of “magnetic particles–PCT antibody–PCT–acridinium ester PCT antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a PCT in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum PCT concentration.

Specifications

Catalog no. SKT-025
Target Procalcitonin
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.040 ng/mL.
Dynamic Range 0.040 ng/mL to 100.0 ng/mL
Total Incubation Time 5 Minutes
Sample Type Serum
Sample Volume 30 µL
Storage Temperature 2-8 °C

Selected Literature


​​​​​​​1. Gendrel D, Bohuon C. Procalcitonin as a marker of bacterial infection. Pediatr InfectDis J 2000:19:679-688.
​​​​​​​2. Becker KL, Nyl én ES, White JC, M uller B,Snider RH. Procalcitonin and the CalcitoninGene Family of Peptides in Inflammation,Infection, and Sepsis: A Journey fromCalcitonin Back to Its Precursors. J ClinEndocrinol Metab 2004;89(4):1512-1525

For in-vitro diagnostic use.
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