Human Creatine kinase-MB CLIA Kit


​​​​​​​Human Creatine kinase-MB CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Creatine kinase-MB concentration in serum.
For in-vitro diagnostics purposes only


The Human Creatine kinase-MB (CK-MB) CLIA Kit is designed, developed, and produced for the quantitative measurement of human CK-MB level in serum samples. The CK-MB assay is a two-site sandwich assay to determination the level of Creatine kinase-MB.
​​​​​​​Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the CK-MB in the form of “magnetic particles–CK-MB antibody–CK-MB– acridinium ester CK-MB antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a CK-MB in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum CKMB concentration..


Catalog no. SKT-024
Target Creatine kinase-MB
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.600 ng/mL
Dynamic Range 0.600 ng/mL to 300.0ng/mL
Total Incubation Time 15 Minutes
Sample Type Serum
Sample Volume 20 µL
Storage Temperature 2-8 °C

Selected Literature

​​​​​​​1. Liu Yu, Zhou Rongbin. Application of colloidal gold immunochromatography to detect myocardial markers in the diagnosis of acute myocardial infarction.Chinese journal of general medicine, 2011,14 (68) : 1903-1904.
​​​​​​​2. Zhang JH, Zhang LL, Zu YQ.The value of three myocardial markers in the early diagnosis of acute myocardial infarction. Chinese journal of pathogen biology, 2012,7 (6) : 461-463.
For in-vitro diagnostic use.