Human C-peptide CLIA Kit

Description

​​​​​​​Human C-peptideCLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitativemeasurement of human C-peptide concentration in serum.

Background

The Human C-peptide CLIA Kit is designed, developed, and produced for the quantitative measurement of human Cpeptide level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of C-peptide.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the Cpeptide in the form of “magnetic particles–C-peptide antibody– C-peptide–acridinium ester C-peptide antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a Cpeptide in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum C-peptide concentration.
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Selected Literature

​​​​​​​1. Sperof FL, Glass RH, Kase NG, editors. Clinical gynecologic endocrinology and infer tility. 4th ed. Baltimore: Williams and Wilkins, 1989, 91–119.
2. Mohr IJ, Clark R, Sun S, et al. Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. Science, 1990, 250(4988): 1694-1699.
​​​​​​​3. Lorick KL, Jensen JP, Fang S, et al. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. ProcNatlAcadSci U S A., 1999, 96(20): 11364-11369.

For in-vitro diagnostic use.