SKT-020R

Human Testosterone CLIA Kit​​​​​​​

Description

Human Testosterone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human testosterone concentration in serum.
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​​​​​​​For research use only. Not for use in diagnostics procedures.

Background


The Human Testosterone CLIA Kit is designed, developed, and produced for the quantitative measurement of human Tes level in serum samples. The assay utilizes competition law for testing. The calibrators, controls, or sample, monoclonal antibody to Tes labeled with microparticles are mixed and incubated, forming an antigen-antibody immuno-complex.Acridinium ester labeled Tes are added and incubated. Acridinium ester labeled Tes analog unbound to the sample binds to the Magnetically microgranular Tes monoclonal antibody, After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of Tes present in the sample. The test result is automatically calculated by the system according to the working curve.
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Specifications

Catalog no. SKT-020R
Target Testosterone
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD ≤0.100 ng/mL
Dynamic Range 0.100ng/mL to 16.00ng/mL
Total Incubation Time 10 minutes
Sample Type Serum
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature


​​​​​​​1.Matsumoto AM, BremnerWJ.Serum testosterone assays accuracy matters.JClinEndocrMetab, 2004, 89(2):520-524.
2.Rinaldi S, Geay A, DéchaudH,etal.Validity of free testosterone and free estradiol determinationsin serum samples from postmenopausal women bytheoretical calculations.CancerEpidemBiomar,2002, 1065–1071.
3.Pardridge WM, Mietus LJ, Frumar AM, et al.Effects of human serum on transport of testosterone and estradiol into rat brain. Am J Physiol,1980,239(1):E103-E108.
For research use only. Not for use in diagnostics procedures.