Human Luteinizing Hormone CLIA Kit


Human Luteinizing Hormone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Luteinizing Hormone concentration in serum.​​​​​​​
For in-vitro diagnostics purposes only


The Human Luteinizing Hormone CLIA Kit is designed, developed, and produced for the quantitative measurement of human LH level in serum samples. The assay utilizes a twosite “sandwich” technique with two antibodies that bind to different epitopes of LH.
​​​​​​​Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the LH in the form of “magnetic particles–LH antibody–LH–acridinium ester LH antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a PRL in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum LH concentration.


Catalog no. SKT-017
Target Luteinizing Hormone
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.200 mIU/mL
Dynamic Range 0.200 mIU/mL to 250.0mIU/mL
Total Incubation Time 10 Minutes
Sample Type Serum
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature

​​​​​​​1. Johnson MR, Carter G , Grint C .Relationship between ovarian steroids gonadotropin and rela ting during the menstrual cycle .ActaEndocrinol , 1993, 129(2):121-125.
2. Marina LV, Ivović M, Tančić-GajićM.Luteinizing hormone and insulin resistance in menopausal patients with adrenal incidentalomas : The cause-effect relationship ClinEndocrinol (Oxf ), 2018, 88 (4): 541-548.
​​​​​​​3. Casarini L, Santi D, SimoniM.'Spare' l uteinizing h ormone r eceptors: f acts and f iction.TrendsEndocrinolMetab , 2018, 29 (4): 208-217.
For in-vitro diagnostic use.