KTR-849

Quantitative Fecal Calprotectin ELISA Kit

Description


This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative determination of human calprotectin (neutrophil cytoplasmic protein A100A8/A9) levels in stool samples. This test is useful for detecting inflammatory bowel disease (IBD) such as ulcerative colitis and Crohn's disease.
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For research use only. Not for use in diagnostics procedures.

Specifications

Catalog no. KTR-849
Target Calprotectin (S100A8/S100A9)
Species Human
Method Sandwich ELISA
Tests Per Kit 96 tests
Detection Colorimetric
Sensitivity / LLOD 2.5 ng /mL
Dynamic Range 0 - 2000 µg/mL
Total Incubation Time 2 hours
Sample Type Stool
Sample Volume 50 µL
Storage Temperature 2-8 °C

Background


Calprotectin consists of mammalian proteins S100A8 and S100A9 and is a 24 kDa dimer1 . It is secreted during the inflammatory response in the intestinal lumen through leukocyte shedding, active secretion, cell disturbance, and cell death. Thus, elevated fecal calprotectin levels are correlated with migration of neutrophils into the intestinal mucosa

Quantitative determination of fecal calprotectin is an indication of the severity of bowel inflammation. High levels of calprotectin in stool are associated with an increased risk of relapse in patients with inflammatory bowel disease (IBD). Low stool calprotectin levels correlate well with a low risk for intestinal allograft injection. Clinical applications may include aiding in the diagnosis of ulcerative colitis, inflammatory bowel diseases (IBD), irritable bowel syndrome (IBS), and Crohn’s disease.

​​​​​​​This assay uses specific monoclonal antibodies to ensure that only calprotectin is detected.
For research use only. Not for use in diagnostics procedures.