Human Neuron Specific Enolase ELISA Kit


This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative determination of human neuron specific enolase (NSE) levels in patient serum samples. This test may be used as an aid for detecting neuroendocrine differentiated tumors in patients, such as small cell lung cancer, neuroblastoma, melanoma, and seminoma, as well as injury to the central nervous system. 
For research use only. Not for use in diagnostics procedures.


The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolyase) exists as several dimeric isoenzymes (αα, αβ, ββ, γγ) composed of three distinct subunits: α, β, γ. Three isoenzymes are found in the human brain: αα, αβ, and γγ. The heterologous αγ isoenzyme and the homologous γγ isoenzyme are known as neuron-specific enolases (NSE), as these isoenzymes initially were detected in neurons and neuroendocrine cells. This ELISA detects both the αγ and γγ forms by using monoclonal antibodies specific to the γ enzyme subunit.
NSE levels are low in the normal healthy population, as well as in people with benign disease. Lung cancer is one of the most common cancer forms with incidences of about 50-100 per 100,000 people. Approximately 20% of lung cancers are of the small cell variety. NSE has been shown to be a valuable tumor marker of neuroendocrine origin, particularly in small cell lung cancer and neuroblastoma. 


Catalog no. KTR-817
Target Neuron Specific Enolase (NSE, enolase 2, ENO2, γ-enolase)
Species Human
Method Sandwich ELISA
Tests Per Kit 96 tests
Detection Colorimetric
Sensitivity / LLOD 5 ng /mL
Dynamic Range 0 - 178 ng/mL
Total Incubation Time 1 hour, 10 minutes
Sample Type Serum
Sample Volume 10 µL
Storage Temperature 2-8 °C
For research use only. Not for use in diagnostics procedures.