Human Fetuin-A ELISA Kit


This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative determination of human fetuin-A, also known as alpha-2-HS glycoprotein (AHSG), in serum, plasma, cell culture supernatant, tissue extraction, and urine. The measurement of fetuin-A in serum or plasma aids in the diagnosis of some cancers and genetically inherited deficiencies of this serum protein. 
For research use only. Not for use in diagnostics procedures.


Catalog no. KTR-800
Target Fetuin-A / alpha-2-HS glyocoprotein / AHSG
Species Human
Method Sandwich ELISA
Tests Per Kit 96 tests
Detection Colorimetric
Sensitivity / LLOD 5 ng/mL
Dynamic Range 0 - 370 ng/mL
Total Incubation Time 2 hours, 50 minutes
Sample Type Serum, plasma, urine, cell culture supernatant, tissue extraction
Sample Volume 10 µL
Storage Temperature 2-8 °C


Fetuin-A, also known as alpha-2-HS glycoprotein, is a 59 kDa glycoprotein that consists of two amino-terminal cystatin domains and a smaller carboxy-terminal domain. It is synthesized by the liver and secreted into blood stream, where its concentration in adult mammals ranges from 0.5 – 1.0 g/L. Fetuin-A occurs with high concentration in serum during fetal life and is involved in protease inhibitory activities and development-associated regulation of calcium metabolism and osteogenesis. It accumulates in bones and teeth as a major fraction of noncollagenous bone proteins. Biological studies have demonstrated that fetuin-A is the major calcification inhibitor found in circulation, where it interferes with calcium salt precipitation.
Recent studies have indicated that fetuin-A levels drop in uremic patients on hemodialysis in comparison to normal healthy controls. The low fetuin-A level in patients with chronic kidney failure strongly associates with higher cardiovascular mortality. On the other hand, it has been demonstrated that a higher than normal serum fetuin-A in an older population is associated with diabetes, which is independent from other markers of insulin resistance. Furthermore, a higher fetuin-A level may be an independent risk marker of patients with cardiovascular diseases.

Selected Literature

  1. Lim P, Collet JP, Moutereau S, Guigui N, Mitchell-Heggs L, Loric S, Bernard M, Benhamed S, Montalescot G, Randé JL, Guéret P. Fetuin-A is an independent predictor of death after ST-elevation myocardial infarction. Clin Chem. 2007 Oct;53(10):1835-40. Epub 2007 Aug 16
  2. Stenvinkel P, Wang K, Qureshi AR, Axelsson J, Pecoits-Filho R, Gao P, Barany P, Lindholm B, Jogestrand T, Heimbürger O, Holmes C, Schalling M, Nordfors L. Low fetuin-A levels are associated with cardiovascular death: Impact of variations in the gene encoding fetuin. Kidney Int. 2005 Jun;67(6):2383-92.
  3. Sato H, Kazama JJ, Wada Y, Kuroda T, Narita I, Gejyo F, Gao P, Yamashita H. Decreased levels of circulating alpha2-Heremans-Schmid glycoprotein/Fetuin-A (AHSG) in patients with rheumatoid arthritis. Intern Med. 2007;46(20):1685-91. Epub 2007 Oct 15.
For research use only. Not for use in diagnostics procedures.