This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative measurement of human adrenocorticotropic hormone in EDTA-plasma. This kit is useful for detecting elevated and deficient ACTH levels.
Indications for use:
Patient may have higher than normal levels of ACTH with
  • Addison's disease / primary adrenal insufficiency
  • Congentical adrenal hyperplasia
  • Cushing's syndrome / Cushing's disease
Patients may have a lower than normal level of ACTH with
  • Hypopituitarism and/or secondary adrenal insufficiency
  • Adrenal gland tumor
  • Other tumors that produce cortisol
This ACTH ELISA kit is for in-vitro diagnostic use.


ACTH is a 39-residue polypeptide with a molecular weight of 4540 Da. It is secreted from corticotropes in the anterior lobe (adenohypophysis) of the pituitary gland in response to cortocotrop-releasing hormone (CRH) released by the hypothalamus. ACTH is synthesized from pre-pro-opiomelanocortin (pre-POMC) The removal of the signal peptide during translation produces the 241-amino acid polypeptide POMC, which undergoes a series of post-translational modifications, such as phorphorylation and glycosylation, before it is proteolytically cleaved by endopeptidases to yield various polypeptide fragments with varying physiological activity.
ACTH is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress. It stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal gland. ACTH acts by binding to cell surface ACTH receptors, which are located primarily on adrenocortical cells of the adrenal cortex.


Catalog no. KT-882
Target Adrenocorticotropic Hormone (ACTH)
Species Human
Method Sandwich ELISA
Tests Per Kit 96 tests
Detection Colorimetric
Sensitivity / LLOD 1 pg /mL
Dynamic Range 0 - 416 ng/mL
Total Incubation Time 2 hour, 20 minutes
Sample Type EDTA-plasma
Sample Volume 200 µL
Storage Temperature 2-8 °C
For in-vitro diagnostic use.