Vitamin B12 ELISA Kit


This enzyme-linked immunosorbent assay (ELISA) kit is intended for the quantitative determination of Vitamin B12 levels in human plasma or serum samples.
This kit is for in vitro diagnostic use only.


This ELISA is designed, developed and produced for the quantitative measurement of Vitamin B12 in human plasma or serum samples. Assay calibrators and patient samples are added directly to wells of a microplate that is coated with an anti-Vitamin B12 monoclonal antibody. After the first incubation period, the antibody on the wall of the microtiter well captures sample Vitamin B12 and HRPconjugated Vitamin B12 in a competitive nature. Excessive amounts of unbound proteins and HRP-conjugated Vitamin B12 in each microtiter well are washed away. For the detection of the captured sample Vitamin B12, the well is incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the complex bound to the Vitamin B12 on the wall of the microtiter well is directly proportional to the amount of Vitamin B12 in the sample. A calibrator standard curve is generated by plotting the absorbance versus the Vitamin B12 concentration for each calibrator on point-to-point or cubical scales or 4 parameter curve fits. The concentration of Vitamin B12 in test samples is determined directly from this standard curve.

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SKT-070 Human Vitamin B12 CLIA Kit ​​​​​​​


Catalog no. KT-716
Target Vitamin B12
Species Human
Method Competitive ELISA
Tests Per Kit 96 tests
Detection Colorimetric
Sensitivity / LLOD 8.630 pg/mL
Dynamic Range
Total Incubation Time 1 hours, 30 minutes
Sample Type serum, plasma
Sample Volume 100 µL
Storage Temperature 2-8 °C
For in-vitro diagnostic use.