Fecal Quantitative Human Fecal Occult Blood CLIA Kit


This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative determination of human hemoglobin levels in fecesusing the ECL100 or ECL25 Immunoassay analyzer. This assay exclusively measures human hemoglobin without cross-reaction to animal blood.  The test is useful for detecting the severity of gastrointestinal bleeding and in the aid of screening for colorectal adenoma/polyps and cancer, as well as other inflammatory bowel diseases (Crohn’s disease, Ulcerative Colitis, etc.).
For research use only. Not for use in diagnostics procedures.


This CLIA is designed, developed, and produced for the quantitative measurement of q-FOB level in fecal samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of hemoglobin.

Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. Simultaneously, anacridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles – biotin hemoglobin antibody –hemoglobin – acridinium ester hemoglobin antibody”.

​​​​​​​The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL25 analyzer. The relative light units (RLU) are proportional to the concentration of hemoglobin in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve and reported in serum hemoglobin concentration.

Related Products

​​​​​​​KTR-850 Quantitative Fecal Occult Blood Test Kit
30210 q-FOB™ Fecal Sample Collection Device
KTR-313 Immunochemical Fecal Occult Blood Rapid Test Kit
KTR-313s Immunochemical Fecal Occult Blood Strip Test Kit
KTR-111 FOB Cassette Test Kit


Catalog no. CL0850R
Target Fecal Occult Blood (q-FOB)
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 4.17µg/g
Dynamic Range 0 - 1019 µg/g
Total Incubation Time <25 minutes after loading the test
Sample Type Stool
Sample Volume 1-2 mL liquid stool sample or 1-5 g solid stool sample.
Storage Temperature 2-8 °C

Selected Literature

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For research use only. Not for use in diagnostics procedures.