Fecal Cryptosporidium parvum Antigen CLIA Kit


This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative detection of Cryptosporidium parvum (Crypto) antigen in feces using the ECL-100 or ECL-25. This assay is a useful tool in the aid of diagnosis of active C. parvum infection in acute or chronic diarrhea.

​​​​​​​This kit is for in vitro diagnostic use only.


This CLIA is designed, developed, and produced for the quantitative measurement of Cryptosporidium parvum in fecal samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Cryptosporidium parvum. Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. An acridinium ester conjugated antibody and a biotin conjugated antibody are added. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles – biotin anti-C. parvum antibody –C. parvum – acridinium ester anti-C. parvum antibody”. The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL-25 analyzer. The relative light units (RLU) are proportional to the concentration of C. parvum in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve and reported in Units per mL concentration.


Catalog no. CL0814
Target Cryptosporidium parvum
Species N/A
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.74 U/mL
Dynamic Range 0 - 82.0 U/mL
Total Incubation Time < 20 minutes
Sample Type Stool
Sample Volume 1-2 mL liquid stool sample or 1- 5 grams solid sample
Storage Temperature 2-8 °C

Selected Literature

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For in-vitro diagnostic use.