Fecal Giardia lamblia Antigen CLIA Kit



This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative detection of Giardia lamblia antigen in feces using the ECL100 or ECL25. This assay is a useful tool in the diagnosis of active Giardia lamblia infection in acute or chronic gastroenteritis.
​​​​​​​For research use only. Not for use in diagnostics procedures.


This CLIA is designed, developed, and produced for the quantitative measurement of Giardia lamblia in fecal samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Giardia lamblia. Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. An acridinium ester conjugated antibody and a biotin conjugated antibody are added. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles – biotin antiGiardialamblia antibody –Giardialamblia – acridinium ester anti-Giardia lamblia antibody”. The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL-25 analyzer. The relative light units (RLU) are proportional to the concentration of Giardia in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in Units per mL concentration.


Catalog no. CL0813R
Target Giardia lamblia Antigen
Species N/A
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.39 U/mL
Dynamic Range 0 - 400 U/mL
Total Incubation Time < 25 minutes
Sample Type Stool
Sample Volume 1- 5 grams solid sample/ 1-2 mL liquid stool sample
Storage Temperature 2-8 °C

Selected Literature

1: Ungar BL, Yolken RH, Nash TE, Quinn TC.  Enzyme-linked immunosorbent assay for the detection of Giardia lamblia in fecal specimens. J Infect Dis. 1984 Jan;149(1):90-7.
2: Rosenblatt JE, Sloan LM, Schneider SK.  Evaluation of an enzyme-linked immunosorbent assay for the detection of Giardia lamblia in stool specimens. Diagn Microbiol Infect Dis. 1993 May-Jun;16(4):337-41.
For research use only. Not for use in diagnostics procedures.