Human Pepsinogen I CLIA Kit


This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative determination of human pepsinogen I levels in serum using the ECL100 or ECL-25 Immunoassay analyzer. Kit would be a useful tool in the aid of diagnosing the functional states of acid gastric mucosa.
For research use only. Not for use in diagnostics procedures.


This CLIA is designed, developed, and produced for the quantitative measurement of human pepsinogen I level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of pepsinogen I.

Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. Simultaneously, an acridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles – biotin pepsinogen I antibody – pepsinogen I – acridinium ester pepsinogen I antibody”.
​​​​​​​The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL-25 analyzer. The relative light units (RLU) are proportional to the concentration of pepsinogen I in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum pepsinogen I concentration.


Catalog no. CL0810R
Target Pepsinogen I
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.26 ng/mL
Dynamic Range 0 - 289.9 ng/mL
Total Incubation Time < 25 minutes after loading the test
Sample Type Serum
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature

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For research use only. Not for use in diagnostics procedures.