Human Carboxylated Osteocalcin (1-43/49) CLIA Kit


This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative determination of human osteocalcin levels in serum or plasma using the ECL100 or ECL-25 Immunoassay analyzer.) The kit is intended for the quantitative determination of both human carboxylated osteocalcin (1-49) and carboxylated osteocalcin (1-43) (also referred as N-terminal & mid-regional osteocalcin) levels in test samples. This test is useful for assessing the bone formation activity or osteoblast activity in patients associated with changes in the rate of bone turnover in metabolic bone disease, such as osteoporosis, primary hyperparathyroidism, hyperthyroidism, Paget’s disease, and renal osteodystrophy.
It is for in-vitro diagnostics use only.


This CLIA is designed, developed, and produced for the quantitative measurement of human osteocalcin level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of osteocalcin.

Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. Simultaneously, anacridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles – biotin osteocalcin antibody –osteocalcin – acridinium ester osteocalcin antibody”.
​​​​​​​The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL-25 analyzer. The relative light units (RLU) are proportional to the concentration of osteocalcin in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve and reported in serum osteocalcin concentration.


Catalog no. CL0809
Target Osteocalcin (1-43/49)
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 2.1 ng/mL
Dynamic Range 0 - 55.8 ng/mL
Total Incubation Time < 25 minutes after loading the test
Sample Type Serum or plasma
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature

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For in-vitro diagnostic use.