CL0012R

Fecal Secretory IgA CLIA kit

Description

This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative determination of Secretory IgA levels in feces using the ECL100 or ECL25 Immunoassay analyzer.

Immunoglobulin A, or IgA, is the second most abundant type of immunoglobulin found in humans. There are two subclasses of IgA; IgA1, which is a monomeric form found in serum, and IgA2, which is a polymeric form found in exocrine secretions such as intestinal mucus, saliva, tears, and breast milk. Secretory IgA (sIgA) primarily belongs in the IgA2 subclass as a dimer that is covalently linked through a J chain, and additionally joined together by a secretory component, which allows for the molecule to cross epithelial surfaces. Secretory IgA plays an incredibly important role in mucosal immunity, and works in immune exclusion by binding to foreign particles such as bacterial and viral antigens, preventing them from attaching to mucosal cells, and promoting the elimination of these antigens by mucociliary functions or peristalsis. Secretory IgA helps to maintain homeostasis in the body, and when there is a sIgA deficiency, it can lead to immune dysregulation and deficiency. Conversely, the production of sIgA can be upregulated when inflammation or infection is occurring, allowing it to be used as an indicator of current gut health.

For research use only. Not for use in diagnostics procedures.

Background


This CLIA is designed, developed, and produced for the quantitative measurement of Secretory IgA in fecal samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different paratopes of Secretory IgA. Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. Acridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody as well as an immune-complex in the form of “magnetic particles – biotin Secretory IgA antibody –Secretory IgA– acridinium ester Secretory IgA antibody”. The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL25 analyzer. The relative light units (RLU) are proportional to the concentration of Secretory IgA in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in Fecal Secretory IgA concentration. 

Specifications

Catalog no. CL0012R
Target Fecal Secretory IgA
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 17.04µg/g
Dynamic Range <510.00 - >2010.00 µg/g
Total Incubation Time <30 min
Sample Type Stool
Sample Volume 1-2 mL
Storage Temperature 2-8 °C

Selected Literature

1. Sapkota, A. (2023, August 3). 5 types of immunoglobulins: IGG, IGM, IGA, IGD, and IGE. Microbe Notes. https://microbenotes.com/antibody/
2. Tankeshwar, A., & Tankeshwar, A. (2023, December 29). IGA antibodies: structure, properties, and functions. Microbe Online. https://microbeonline.com/immunoglobulin iga-structure-functions/
3. Mantis NJ, Rol N, Corthésy B. Secretory IgA's complex roles in immunity and mucosal homeostasis in the gut. Mucosal Immunol. 2011 Nov;4(6):603-11. doi: 10.1038/mi.2011.41. Epub 2011 Oct 5. PMID: 21975936; PMCID: PMC3774538.
4. Conrey PE, Denu L, O'Boyle KC, Rozich I, Green J, Maslanka J, Lubin JB, Duranova T, Haltzman BL, Gianchetti L, Oldridge DA, De Luna N, Vella LA, Allman D, Spergel JM, Tanes C, Bittinger K, Henrickson SE, Silverman MA. IgA deficiency destabilizes homeostasis toward intestinal microbes and increases systemic immune dysregulation. Sci Immunol. 2023 May 26;8(83):eade2335. doi: 10.1126/sciimmunol.ade2335. Epub 2023 May 26. PMID: 37235682; PMCID: PMC11623094.
​​​​​​​5. Li, Yue, Jin, Liang, Chen, Tongxin, The Effects of Secretory IgA in the Mucosal Immune System, BioMed Research International, 2020, 2032057, 6 pages, 2020. https://doi.org/10.1155/2020/2032057 6. Pietrzak B, Tomela K, Olejnik-Schmidt A, Mackiewicz A, Schmidt M. Secretory IgA in Intestinal Mucosal Secretions as an Adaptive Barrier against Microbial Cells. Int J Mol Sci. 2020 Dec 4;21(23):9254. doi: 10.3390/ijms21239254. PMID: 33291586; PMCID:
For research use only. Not for use in diagnostics procedures.
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