CL0011R

Fecal Lactoferrin CLIA Kit

Description

This Chemiluminescence Immunoassay (CLIA) kit is intended for the quantitative determination of Lactoferrin levels in feces using the ECL100 or ECL25 Immunoassay analyzer.

Lactoferrin is an iron-binding protein that can be secreted by non-specific inflammatory cells called neutrophils1. It is a glycoprotein consisting of approximately 700 amino acids, and can be found in mucosal secretions, plasma, tears, milk, and saliva. Fecal Lactoferrin serves as a biomarker for intestinal inflammation, as the lactoferrin concentration in the gastrointestinal tract is shown to increase with the severity of inflammation. Lactoferrin tests are particularly useful when determining if gastrointestinal symptoms are functional, such as with irritable bowel syndrome, or due to infection or inflammation, such as with inflammatory bowel disease1. Fecal lactoferrin tests are non-invasive ways to indicate the presence of several conditions such as ulcerative colitis, Crohn’s disease, and inflammatory bowel disease.
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For research use only. Not for use in diagnostics procedures.

Background


This CLIA is designed, developed, and produced for the quantitative measurement of Lactoferrin in fecal samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Lactoferrin.

Assay calibrators, controls, or patient samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. Acridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody as well as an immune-complex in the form of “magnetic particles – biotin Lactoferrin antibody –Lactoferrin– acridinium ester Lactoferrin antibody”.

The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 or ECL25 analyzer. The relative light units (RLU) are proportional to the concentration of Lactoferrin in the sample. The amount of analyte in the sample is determined from a stored, multi point calibration curve and reported in FecalLactoferrin concentration.

Specifications

Catalog no. CL0011R
Target Fecal Lactoferrin
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.56 µg/g
Dynamic Range 0.00 - 7.25 µg/g
Total Incubation Time <30 min
Sample Type Stool
Sample Volume 1-5 g (solid), 1-2 mL (liquid)
Storage Temperature 2-8 °C

Selected Literature

1. Abraham BP. Fecal Lactoferrin Testing. Gastroenterol Hepatol (N Y). 2018 Dec;14(12):713-716. PMID: 30804718; PMCID: PMC6383158.
2. Assche GV. Fecal biomarkers for the diagnosis and management of inflammatory bowel disease. Gastroenterol Hepatol (N Y). 2011 Jun;7(6):396-8. PMID: 21869871; PMCID: PMC3151412.
​​​​​​​3. Liu F, Lee SA, Riordan SM, Zhang L and Zhu L (2020) Global Studies of Using Fecal Biomarkers in Predicting Relapse in Inflammatory Bowel Disease. Front. Med. 7:580803. doi: 10.3389/fmed.2020.580803
For research use only. Not for use in diagnostics procedures.
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