Hepatitis A Virus IgM ELISA Kit

Description

This kit is an enzyme-linked immunosorbent assay (ELISA, IgM Capture Method) for the qualitative determination of human IgM antibody against Hepatitis A Virus in human plasma and serum. 

This kit is for research use only.

Background

Hepatitis A (formerly known as infectious hepatitis) is an acute infectious disease of the liver caused by the Hepatitis A virus, which is most commonly transmitted by the fecal-oral route via contaminated food or drinking water. Every year, approximately 10 million people worldwide are infected with the virus. The time between infection and the appearance of symptoms (the incubation period), is between two and six weeks with the average incubation period at 28 days. Hepatitis A does not have a chronic stage and does not cause permanent liver damage. Following infection, the immune system makes antibodies against the hepatitis A virus which confers immunity against future infection. The disease can be prevented by vaccination and hepatitis A vaccine has been proven effective in controlling outbreaks worldwide.


The Hepatitis virus (HAV) is a picornavirus; it is non-enveloped and contains a single-stranded 27 nm RNA packaged in a protein shell. There is only one type of the virus.


Although the virus is excreted in the feces towards the end of the incubation period, specific diagnosis is made through the detection of Hepatitis A virus specific IgM antibodies in blood. IgM antibody is present in blood following an acute hepatitis A infection. It is detectable one to two weeks after the initial infection and persists for years. The presence of IgM antibody in the blood means that the acute stage of the illness is past and the person is immune to further infection. IgM antibody to HAV is also found in the blood following vaccination and tests for immunity to the virus are based on the detection of this antibody. During the acute stage of infection, the liver enzyme alanine transferase (ALT) is present in the blood at levels much higher than is normal. The enzyme comes from the liver cells that have been damaged by the virus.

Related Kits

This kit is part of the Epitope Diagnostics ToRCH panel.

 

Specifications

 

Catalog no.

Target:

Species

Method

Tests Per Kit

Detection

Total Incubation Time

Sample Type

Sample Volume

Storage Temperature

 

KTR-868

Hepatitis A Virus 

Human

Sandwich ELISA

96 tests

Colorimetric

2 hours

Serum, EDTA-plasma

10 µL

2-8 °C

For research use only. Not for use in diagnostic procedures.