Human Pepsinogen I ELISA Kit


This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative measurement of human pepsinogen I levels in serum. Pepsinogen I levels are a useful tool in the determination the functional states of acid-secreting gastric mucosa. 

This pepsinogen I ELISA kit is for in-vitro diagnostic use.


Pepsinogen consists of a single polypeptide chain of 375 amino acid residues with an average molecular weight of 42 kDa. Pepsinogen I is synthesized at gastric cheif cells and mucous neck cells, while pepsinogen II is also produced by clear mucous cells of antrum, etc. The clinical applications of measuring pepsinogen I and II are a useful aid in the diagnosis of severe atrophic gastritis and stomach cancer. It has been suggested that the measurement of serum pepsinogens serve as a "serological biopsy" for predicting the presence of atrophic gastritis or superficial gastritis. 

Atrophic Gastritis: It has been found the serum pepsinogen I levels falling lower than 20 ng/ml was highly specific for severe atrophic gastritis. It was also observed that serum pepsinogen I levels fell with increasing severity of mucosal damage in such cases. The diagnostic sensitivity and specificity of serum pepsinogen I levels for advanced atrophic corpus gastritis are about 92% and 90% respectively. On the other hand, the decrease in serum pepsinogen I levels in patients with pernicious anemia and atrophic gastritis was found to be associated with normal or raised pepsinogen II levels. Therefore, a pepsinogen I/pepsinogen II ratio is significantly lower than those with superficial gastritis or normal remnant mucosa.

Stomach Cancer: Low serum pepsinogen I levels were found in patients with gastric cancer, with a threefold higher incidence. Other studies have concluded that low serum pepsinogen I levels may identify persons at increased risk for intestinal types of stomach cancer.

Duodenal Ulcers: A low serum pepsinogen I level can exclude a diagnosis of duodenal ulcer. Although a high pepsinogen I level has less clinical use for establishing this diagnosis, the combination of hypergastinemia and a highly evelated serum pepsinogen I level strongly suggests the possibility of Zollinger-Ellison syndrome.

Related Kits

KT-811 Human Pepsinogen II ELISA Kit




Catalog no.




Tests Per Kit


Sensitivity / LLOD

Dynamic Range

Total Incubation Time

Sample Type

Sample Volume

Storage Temperature



Pepsinogen I


Sandwich ELISA

96 tests


0.1 ng/mL

0 - 300 ng/mL

1 hour, 20 minutes


25 µL

2-8 °C

For in-vitro diagnostic use.